Bacteriocins from Lacticaseibacillus paracasei CNCM I-5369: anti-Escherichia coli activity, original export system, potential medical application
Françoise Coucheney
Université de Lille, Villeneuve d'Ascq, France
A leading approach to combating antibiotic resistance is the development of antimicrobial molecules such as bacteriocins. A better understanding of these peptides produced by bacteria will enable their use in food processing, animal health, and even human health applications. With this in mind, the Lacticaseibacillus paracasei CNCM I-5369 strain, which has a broad spectrum of antimicrobial activity, has been isolated and identified. It is capable of inhibiting Gram-positive and Gram-negative bacteria, including Enterobacterales that are multi-resistant to antibiotics. This bacterium is particularly interesting because it produces five original class II bacteriocins. The genetic organization of these ORFs is atypical. This study highlights the bacteriocin export system in this strain and provides insights into the mode of action of the bacteriocins produced by Lb. paracasei CNCM I-5369. These results contribute to a better understanding of bacteriocins, opening up both fundamental and applied perspectives in health.